Table 2.
Summary of binding lifetimes of UvrA-YPet and Mfd-YPet after UV exposure.
| Strain (genotype) | Probe | Treatment | Afast (%) | τfast (s) | Aslow (%) | τslow (s) | kb (s−1) | Exponential fit | Figure |
|---|---|---|---|---|---|---|---|---|---|
| uvrA-YPet Δmfd | UvrA-YPet (chr) | 20 Jm−2 UVC (temporally averaged) | 77 ± 3 | 1.6 ± 0.1 | 23 ± 3 | 13.1 ± 0.6 | 6.2 ± 0.2 | 2 | 5 |
| uvrA-YPet Δmfd | UvrA-YPet (chr) | 20 Jm−2 UVC (0–25 min) | 66 ± 1 | 1.6 ± 0.3 | 34 ± 1 | 9.6 ± 1 | 5.2 ± 0.3 | 2 | 5 |
| uvrA-YPet Δmfd | UvrA-YPet (chr) | 20 Jm−2 UVC (25–50 min) | 80 ± 5 | 1.3 ± 0.1 | 20 ± 5 | 13 ± 1 | 5.6 ± 0.4 | 2 | 5 |
| uvrA-YPet Δmfd | UvrA-YPet (chr) | 20 Jm−2 UVC (50–75 min) | 76 ± 5 | 1.9 ± 0.3 | 24 ± 5 | 15 ± 2 | 6.7 ± 0.3 | 2 | 5 |
| uvrA-YPet Δmfd | UvrA-YPet (chr) | 20 Jm−2 UVC (75–100 min) | 83 ± 3 | 1.5 ± 0.3 | 17 ± 3 | 15 ± 4 | 6.5 ± 0.5 | 2 | 5 |
| uvrA-YPet | UvrA-YPet (chr) | 20 Jm−2 UVC (temporally averaged) | 74 ± 2 | 1.5 ± 0.1 | 26 ± 2 | 9.9 ± 0.4 | 5.5 ± 0.1 | 2 | 5 |
| uvrA-YPet | UvrA-YPet (chr) | 20 Jm−2 UVC (0–25 min) | 71 ± 0.1 | 2.6 ± 0.7 | 29 ± 0.1 | 12 ± 3 | 4.8 ± 0.1 | 2 | 5 |
| uvrA-YPet | UvrA-YPet (chr) | 20 Jm−2 UVC (25–50 min) | 70 ± 3 | 2.2 ± 0.4 | 30 ± 3 | 11.6 ± 0.9 | 5.9 ± 0.3 | 2 | 5 |
| uvrA-YPet | UvrA-YPet (chr) | 20 Jm−2 UVC (50–75 min) | 78 ± 2 | 1.4 ± 0.1 | 22 ± 2 | 10.2 ± 0.6 | 5.6 ± 0.2 | 2 | 5 |
| uvrA-YPet | UvrA-YPet (chr) | 20 Jm−2 UVC (75–100 min) | 87 ± 2 | 1.2 ± 0.2 | 13 ± 2 | 8.4 ± 0.6 | 5.8 ± 0.4 | 2 | 5 |
| mfd-YPet | Mfd-YPet (chr) | 20 Jm−2 UVC (temporally averaged) | 100 | 12.0 ± 0.6 | 6.0 ± 0.1 | 1 | 5 | ||
| mfd-YPet | Mfd-YPet (chr) | 20 Jm−2 UVC (0–25 min) | 100 | 13.7 ± 0.8 | 5.2 ± 0.2 | 1 | 5 | ||
| mfd-YPet | Mfd-YPet (chr) | 20 Jm−2 UVC (25–50 min) | 100 | 11.5 ± 0.8 | 6.0 ± 0.2 | 1 | 5 | ||
| mfd-YPet | Mfd-YPet (chr) | 20 Jm−2 UVC (50–75 min) | 100 | 10 ± 1 | 6.9 ± 0.2 | 1 | 5 | ||
| mfd-YPet | Mfd-YPet (chr) | 20 Jm−2 UVC (75–100 min) | 100 | 8 ± 1 | 6.4 ± 0.3 | 1 | 5 |