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. 2020 Mar 20;9(3):36. doi: 10.1038/s41389-020-0220-5

Fig. 2. RNF187 inhibits Hippo signaling in triple negative breast cancer cells.

Fig. 2

a RNF187 depletion increased YAP protein levels in BT549 cells. BT549 cells were transfected with siControl or siRNF187. After 48 h, cells were harvested for western blot analysis. RNF187 and YAP protein levels were determined by Western blot. Actin was used as internal control. b RNF187 depletion increased YAP protein levels in MDAMB231 cells. MDAMB231 cells were transfected with siControl or siRNF187. After 48 h, cells were harvested for western blot analysis. RNF187 and YAP protein levels were determined by Western blot. Actin was used as internal control. c RNF187 over-expression decreased YAP protein levels in BT549 cells. BT549 cells were transfected with Myc-RNF187 or Myc plasmids. After 48 h, cells were harvested for western blot analysis. Myc-RNF187 and YAP protein levels were determined by Western blot. Actin was used as internal control. d RNF187 depletion increased YAP target gene expression in TNBC cells. BT549 cells were transfected with siControl or siRNF187. After 48 h, total RNA was extracted for gene expression analysis. Each group was done in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001 for target gene expression comparison. e RNF187 overexpression decreased YAP target gene expression in TNBC cells. BT549 cells were transfected with Myc-RNF187 or Myc plasmids. After 48 h, total RNA was extracted for gene expression analysis. Each group was done in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001 for target gene expression comparison.