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. 2020 Mar 20;11:1509. doi: 10.1038/s41467-020-15140-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Fractionation pathway of H7 L. inversa extract, starting from fraction F87-1. b Schematic representation of the firefly luciferase construct used to measure readthrough activity. HeLa cells were transfected with an expression vector carrying a firefly luciferase (Fluc) gene consisting of the open-reading frame encoding firefly luciferase interrupted by an intron and a nonsense codon at position 109. c Luciferase activity in HeLa cells transfected with the construct described in b carrying a UGA, UAA, or UAG PTC and incubated with DMSO, H7 extract, one of various H7 fractions, or DAP. The value of each luciferase measurement is presented in the Source Data File. d Readthrough activity on the construct described in b as a function of the G418 or DAP dose. The value of each luciferase measurement is presented in the Source Data File. All data shown in c, d are representative of two independent experiments. Source data are provided as a Source Data file.