Fig. 4. DAP corrects UGA nonsense mutations in vivo.

a Twenty-eight nude mice were injected with Calu-6 or 4T1 cells to promote tumor development. The mice were exposed to DMSO or DAP for about 5 weeks and tumor size was measured at different time after cell injection and plotted on the graph. For each experimental condition, 7 mice were used. b Fourteen nude mice were injected with Calu-6 cells to promote tumor development. The mice were exposed to DMSO or DAP when the average tumor size reached about 0.4 cm³. The arrow indicates the starting time of the exposure to DMSO or DAP. For each experimental condition, 7 mice were used. The tumor growth curve is presented on the left, and the right panel shows the western-blot analysis of p53 protein in the Calu-6-cell tumors of the seven DMSO-exposed and seven DAP-exposed mice. GAPDH was used as loading control. A molecular weight marker is indicated to the left of the gel. The results presented in this figure are representative of two independent experiments. p-values (p) were calculated using Student’s t-test. Source data are provided as a Source Data file.