Table 1.
Micronucleus induction assay.
| Treatment conditions | DAP dose (µM) | Micronucleated cells |
|---|---|---|
| Assay without S9 mix | 0 | 0 |
| 20 | 2 | |
| 40 | 3 | |
| 70 | 1 | |
| Mitomycin (1 µg/ml) | 100 | |
| Assay with S9 mix | 0 | 0 |
| 40 | 0 | |
| 290 | 1 | |
| 580 | 0 | |
| Cyclopho sphamide (6 µg/ml) | 60 |
L5178Y TK+/− mouse lymphoma cells were exposed to increasing doses of DAP (as indicated), DMSO (DAP concentration 0 µM), or mitomycin or cyclophosphamide used as a positive control. 1000 cells were analyzed per condition and the number of micronucleated cells is reported. The experiment was performed in the absence (above) or presence (below) of S9 mix.