Figure 1.

Reduced cytokine and chemokine expression associated with lymphoid follicle formation in male H37Rv infected mice. Females and males were infected via aerosol with H37Rv and lungs were collected at day 152 post infection for (A) anti-B220 staining of PFA-fixed, paraffin-embedded lung tissue sections. Arrows represent B220 positive follicles. Representative micrographs from one mouse out of ten mice per group are shown. Bar = 500 µm. In addition, lungs were collected at day 21 and day 152 post infection for analysis of (B) protein expression of IL-23 and (C) expression of chemokines associated with formation of lymphoid aggregates by qRT-PCR (relative expression (RQ) to housekeeping gene HPRT). Data is presented as mean ± SD (n = 10). Statistical analysis was performed by Student’s t-test. *p ≤ 0.05, **p ≤ 0.01.