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. 2020 Mar 20;10:5142. doi: 10.1038/s41598-020-61503-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bacterial burden in HN878 infected male and female C57BL/6 mice. Females and males were infected via aerosol with a low dose of HN878. CFU were determined in homogenates of lung (A), lymph node (LN; C), and spleen (D) at different time points. (B) PFA-fixed, paraffin-embedded lung tissue sections from day 105 post infection were subjected to ZN staining for detection of HN878 (arrows). Representative micrographs from one mouse out of five mice per group are shown. Bar = 50 µm. (A,C and D) Data is presented as mean ± SD (n = 5). Statistical analysis was performed by Student’s t-test. ***p ≤ 0.001.