Figure 4.

Reduced number of lymphoid aggregates in Mtb infected male C57BL/6 mice. Females and males were infected via aerosol with a low dose of HN878. Lungs were collected at day 105 and PFA-fixed, paraffin-embedded tissue sections were stained with H&E (A; arrows: lymphoid aggregates), or stained with antibodies to detect (B) B220, or (C) CD3. Representative micrographs from one mouse out of five mice per group are shown. Bar = 2 mm (A) and 500 µm (B + C). (D) Quantitative analysis of the number of B220 positive structures shown in (B) per mm² lung tissue from 5 mice per group. Each data point represents one mouse. Statistical analysis was performed by Student’s t-test. *p ≤ 0.05.