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. 2020 Mar 20;11:1481. doi: 10.1038/s41467-020-15284-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Intact HS standards were subjected to DTIMS to define collision cross section (CCS) values. Variant degrees of separation were observed for (a), Disaccharides ΔUA-GlcNAc6S (#2) and ΔUA2S-GlcNAc (#7). b Tetrasaccharides GlcA-GlcNAc6S-GlcA-GlcNAc6S-R₁ (#9) and IdoA-GlcNAc6S-IdoA-GlcNAc6S-R₁ (#10), where R₁ is (CH₂)₅NH₂. c Octasaccharides GlcNAc6S-[IdoA-GlcNAc6S]₃-IdoA-R₂ (#19) and GlcNAc6S-[GlcA-GlcNAc6S]₃-GlcA-R₂ (#20), where R₂ is C₇H₇O. d Decasaccharides GlcNAc6S-[IdoA-GlcNAc6S]₄-IdoA-R₂ (#27) and GlcNAc6S-[GlcA-GlcNAc6S]₄-GlcA-R₂ (#28), where R₂ is C₇H₇O. Full details of structures and CCS values are provided in Supplementary Tables 1 and 2.