Table 1.

Quantification of phospholipid species in human nuclear membranes (hNM) by liquid-state NMR.

Phospholipida	31P-chemical shift (ppm) from literature	31P-chemical shift hNM (ppm)c	% mold
TPP (reference)		−17.95
PC	−0.84 ± 0.01	−0.84 ± 0.01	46 ± 4
EPC	−0.78 ± 0.01	−0.77 ± 0.01	15 ± 2
LPCe	−0.28 ± 0.04	−0.22 ± 0.01	0 ± 1
PI	−0.36 ± 0.03	−0.20 ± 0.06	11 ± 1
SM	−0.07 ± 0.03	−0.10 ± 0.01	3 ± 2
PSg	−0.05 ± 0.01	−0.06 ± 0.01	4 ± 1
PE	0.04 ± 0.10	0.03 ± 0.01	9 ± 2
LPIe	0.10 ± 0.04	0.03 ± 0.01	4 ± 1
CLg	0.18 ± 0.01	0.06 ± 0.01	1 ± 1
PA	0.23 ± 0.04	0.33 ± 0.02	6 ± 1
PGf	0.47 ± 0.01	0.57 ± 0.01	0 ± 1

^aTPP: Triphenylphosphate (internal reference), PC: phosphatidylcholine, EPC: ether phosphatidylcholine, LPC: lysophosphatidylcholine, PI: phosphatidylinositol, LPI: lysophosphatidylinositol SM: sphingomyelin, PS: phosphatidylserine, PE: phosphatidylethanolamine, CL: cardiolipin, PA: phosphatitic acid, PG: phosphatidylglycerol.

^bChemical shifts of individual phospholipids in the MeOD/CDCl₃ (from Kaffarnik et al. and Meneses & Glonek^18,19). Average over the 2 reference values. Reference H₃PO₄ (0 ppm).

^cChemical shifts of individual phospholipids in the nuclear lipid extract mixture (4 mg of phospholipids solubilized in of 500 µL of MeOD/CDCl₃ (1:2) + 30 µl of 0.2 M EDTA-D₂O pH 6). Chemical shifts were referenced with respect to the internal standard TPP: -17.95ppm¹⁸, assignment was performed with ¹H-³¹P 2D NMR (supplementary materials). Values in the column represent the average over 3 different sample extractions over a period of one year.

^dMolar lipid content in the extract as determined from simulated spectra using the DMFIT software (see Materials and Methods and Supplementary Fig. 1). Figures represent the average over 3 NMR sample preparations. Bold stand for the most abundant species.

^eOnly detected once among 3 samples. Assignment by comparison with literature values.

^fOnly detected once among 3 samples.

^gAssignment by comparison with literature values.