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. 2020 Mar 20;10:5163. doi: 10.1038/s41598-020-62136-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of EGCG and nano-EGCG on the colony formation activity in lung cancer cells. (A) Representative images of the anchorage-independent colony formation assay in H1299 and A549 cells. The graphs are the summarized data of the colony formation assays. Colonies >0.5 mm in diameter were counted and displayed in comparison with the control group. Anchorage-dependent colony formation assays of H1299 cells treated with EGCG (B) and nano-EGCG (C). (D) The effects of nano-EGCG on A549 cells anchorage-dependent colony formation abilities were also studied. Colonies >0.8 mm in diameter were counted and displayed in comparison with the control group. Values are reported as means ± SD (n ≥ 3). *P < 0.05 compared with the control group.