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. 2020 Mar 20;10:5163. doi: 10.1038/s41598-020-62136-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoblot analysis revealed the effects of nano-EGCG treatment on AMPK-related pathway gene expression levels. The expression levels of Akt, p-Akt, LKB-1, p-LKB-1, AMPK, p-AMPK, mTOR, p-mTOR, p-P70, and p-4EBP1 proteins in the H1299 cells that were treated with different concentrations of nano-EGCG were determined through western blot analysis. The nanoemulsion without EGCG was used as a control group. GAPDH was used as an internal control for protein loading and transfer. The original blots/gels are presented in Supplementary Information.