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. 2020 Mar 20;10:5163. doi: 10.1038/s41598-020-62136-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Nano-EGCG and AMPK inhibitors on H1299 cell migration. H1299 cells were seeded in the chamber of a 6-cm dish and incubated for 1 day before the addition of Nano-EGCG (2 μM) and AMPK inhibitors (BML-275, 100 nM). The wound healing assay was performed and photographs were taken at 0, 4, and 8 hours after treatment. The total numbers of migrated cells in the denuded zone were counted. These results are representative of two independent experiments performed in triplicate. *P < 0.05 significantly different from the indicated control group at the indicated time. Data are expressed as mean ± SD.