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In silico predictions of metabolic nutrient requirements across multiple Gram-negative species. (A) Nutrients for which a specific auxotrophy was predicted in at least one strain. (B) Top 15 metabolites for which a nonspecific auxotrophy was predicted (see Dataset S2 for the full results). Nutrient requirements were predicted for a total of 54 strains across Escherichia coli, Salmonella, Klebsiella, and Yersinia, with amino acids auxotrophies appearing with the highest frequency.
