Fig. 3.
Lymphocyte infiltration into primary tumors. Primary tumors were removed from 20-wk-old PyMT and PyMTΔ2c44 (Δ2c44) mice. (A) F4/80 (green) and CD11b+ (red) staining, bar = 20 µm; n = 8 to 12 mice per group with 5 sequential slides evaluated per tumor (Student’s t test). (B) FACS-based quantification of primary tumor digests for monocytes, neutrophils, resident macrophages (Res. MO), CD45+ cells, tumor-associated macrophages (TAMs), lymphocytes, T cells, and NKT cells relative to the total cell count (total); n = 6 to 14 animals per group (Student’s t test). **P < 0.01; ***P < 0.001.