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. 2020 Mar 3;117(11):6092–6102. doi: 10.1073/pnas.1921187117

Fig. 3.

Fig. 3.

KLHL14 and BCR subunits are in close proximity in ABC DLBCL cells. (A) Confocal images of Tet repressor-expressing TMD8 cells retrovirally transduced with cDNAs encoding BioID2-HA-tagged KLHL14. Cells were treated with 1 μg/mL of doxycycline (DOX) for 24 h and stained as indicated. (B) Confocal images of TMD8 cells stained as indicated. (C, Top) Confocal images of PLAs (red) showing interaction of induced KLHL14 with IgM (C, Left), CD79A (C, Middle), and CD79B (C, Right) in Tet repressor-expressing TMD8 cells retrovirally transduced with cDNAs encoding BioID2-HA-tagged KLHL14 and treated with DOX (1 μg/mL) for 24 h. (Bottom) Normalized PLA score for each PLA pair. (D) Normalized PLA score after shRNA knockdown of the indicated genes (see Materials and Methods for detail). Error bars represent SEM, and data are representative of three independent experiments.