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. 2020 Mar 3;117(11):6092–6102. doi: 10.1073/pnas.1921187117

Fig. 4.

Fig. 4.

KLHL14 decreases stability of the immature BCR glycoforms in the ER. (A, Left) Western blot analysis of PSA (ER associated, Top)- and RCA (Golgi associated, Bottom)-bound fractions of BCR in Tet repressor-expressing TMD8 cells retrovirally transduced with cDNAs encoding BioID2-HA-tagged KLHL14. Cells were pretreated with DOX (1 μg/mL) for 16 h and treated with CHX (50 μg/mL) for the indicated time points before lysis. Right, quantification of BCR protein levels for PSA (ER associated, Top)- and RCA (Golgi associated, Bottom)-bound fractions. (B, Left) Western blot analysis of PSA (ER associated, Top)- and RCA (Golgi associated, Bottom)-bound fractions of BCR in TMD8-Cas9 cells lentivirally transduced with indicated sgRNAs. Cells were treated with CHX (50 μg/mL) for the indicated time points before lysis. (B, Right) Quantification of BCR protein levels for PSA (ER associated, Top)- and RCA (Golgi associated, Bottom)-bound fractions. Ctrl, nontargeting control. Error bars represent SD of triplicates, and data are representative of three independent experiments.