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. 2020 Mar 2;117(11):5782–5790. doi: 10.1073/pnas.1920200117

Fig. 1.

Fig. 1.

SOX4 potently inhibits glioblastoma cell proliferation and growth. (A) Immunocytochemistry of proliferating glioblastoma cells indicated by Ki67-staining through a time-course analysis. Virus-transduced U251 glioblastoma cells are indicated by the coexpressed GFP. (Scale bar for lower magnification images, 50 µm.) (B) Quantification of Ki67+ glioblastoma cells at the indicated time points (mean ± SEM; n = 3; **P < 0.01, ***P < 0.001, and ****P < 0.0001). (C) Confocal images of glioblastoma cells undergoing DNA replication. BrdU was applied 2 h before immunocytochemistry. GFP expression indicates virus-transduced U251 glioblastoma cells. (Scale bar for lower magnification images, 50 µm.) (D) Quantification of BrdU+ glioblastoma cells at the indicated time points (mean ± SEM; n = 3; ***P < 0.001 and ****P < 0.0001). (E) A time-course analysis of relative cell proliferation by measuring ATP-dependent luminescence (mean ± SEM; n = 6; **P < 0.01 and ****P < 0.0001).