Fig. 4.

SOX4 represses mRNA translation. (A) A schematic diagram of the experimental design. Cells were incubated with puromycin (Puro) for 20 min (m) before lysis for Western blotting (WB). d, day; h, hour. (B) Western blotting analysis of the puromycin-labeled neosynthesized peptides. Ponceau staining and ACTB were used as loading controls. 0, 0 d; 3, 3 d; MW, molecular weight. (C) Quantification of puromycin-labeled neosynthesized peptides after normalization to ACTB expression (mean ± SEM; n = 5; **P < 0.01).