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Effect of RIPC prior to I/R on autophagy protein expression in vitro and in vivo. Western blot analysis of Atg5-Atg12 conjugate in (A) H9c2 cells, (B) rat heart lysate and LC3 protein levels in (C) H9c2 cells, (D) rat heart lysate, and (E) bafilomycin-A1-treated H9c2 cell extracts, expressed as mean ± SEM, fold relative to control; * p < 0.05, ** p < 0.01.
