Table 1.
Primer sequences used for RT-PCR.
| Gene | Primer Sequence | Annealing Temperature |
|---|---|---|
| Cardiotrophin-1 | Forward 5′-AGCCTTCCTGATTTCTCAGGCT-3′ Reverse 5′-AATT TCTGTGTTGGCGCAGTGTGG-3′ |
60 °C |
| LIF | Forward 5′-TGTGGTAGCAGCCGAGTCATAAT-3′ Reverse 5′-CGTGGCTTGTGTGTTCGGTTTCAT-3′ |
60 °C |
| IL-6 | Forward 5′-GCTCGTCGTCGACAACGGCCTC-3′ Reverse 5′-CAAACATGATCTGGGTCATCTTCTC-3′ |
60 °C |
| IL-11 | Forward 5′-CGTGAAGCTGTGTTGTCCTG-3′ Reverse 5′-GCTCCTAGGACTGTCTTCTTC-3′ |
60 °C |
| IL-1β | Forward 5′-CTGTGACTCGTGGGATGATG-3′ Reverse 5′-GGGATTTTGTCGTTGCTTGT-3′ |
60 °C |
| GAPDH | Forward 5′-ATGGGAAGCTGGTCATCAAC-3′ Reverse 5′ GTGGTTCACACCCATCACAA-3′ |
60 °C |