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. 2020 Feb 29;21(5):1669. doi: 10.3390/ijms21051669

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HRMA—Francisella spp. parE. (a) Melt curves for Francisella spp. parE amplicons. (b) Comparison of reference parE sequence with published sequences for the F. philomiragia strains (see Table 9). All F. tularensis strains tested are 98% identical to the reference sequence within this locus. Primer sequences are underlined. The sequence within the red box indicates the 5-nt deletion of interest [34]; mutations/substitutions predicted to increase or decrease amplicon T_ms are highlighted in green or yellow, respectively.