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. 2020 Feb 29;21(5):1669. doi: 10.3390/ijms21051669

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HRMA—B. cereus and B. anthracis parC. (a) Melt curves for B. cereus and B. thuringiensis parC amplicons. (b) Comparison of reference B. anthracis parC sequence with published sequences for the B. cereus (BC) and B. thuringiensis (BT) strains (see Table 13 for accession numbers). Primer sequences are underlined; mutations at the locations shown in red have been documented to cause fluoroquinolone resistance in B. anthracis. Substitutions predicted to increase or decrease amplicon T_ms are highlighted in yellow or green, respectively.

HRMA—B. cereus and B. anthracis parC. (a) Melt curves for B. cereus and B. thuringiensis parC amplicons. (b) Comparison of reference B. anthracis parC sequence with published sequences for the B. cereus (BC) and B. thuringiensis (BT) strains (see Table 13 for accession numbers). Primer sequences are underlined; mutations at the locations shown in red have been documented to cause fluoroquinolone resistance in B. anthracis. Substitutions predicted to increase or decrease amplicon T_ms are highlighted in yellow or green, respectively.