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. 2020 Mar 5;21(5):1802. doi: 10.3390/ijms21051802

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Biological characterization of adipose-derived mesenchymal stem cells (ASCs): (a) Representative flow cytometric plots of immunophenotype. AB+—ASCs were stained with antigen-specific fluorescent antibody. NC—negative control (ASCs were stained with matched fluorescent nonimmune antibody). Canonical ASC markers: CD90, CD105, CD73, CD44, CD29, HLA-ABC. Endothelial marker: CD31. Hematopoietic markers: CD34, CD45. (b) Differentiation capacity evaluation. ASCs were cultured in osteogenic differentiation medium and stained for alkaline phosphatase and alizarin red S. ASCs were cultured in adipogenic differentiation medium and stained with Oil red O.