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. 2020 Feb 27;21(5):1634. doi: 10.3390/ijms21051634

Figure 3.

Figure 3

Phylogenetic analysis of REVEILLEs (RVEs) and the expression patterns of PbRVEs in ‘Red Zaosu’ pear fruit skin. (a) Phylogenetic analysis of RVE proteins from pear and A. thaliana. The type I proteins are AtRVE7, AtRVE7-Like, PbRVE7, AtRVE1, AtRVE2, PbRVE1a and PbRVE1b; the type II proteins are AtRVE3, AtRVE5, PbRVE3a, PbRVE3b, AtRVE6, PbRVE6, PbRVE8, AtRVE4 and AtRVE8. (b) The expression patterns of PbRVEs in ‘Red Zaosu’ fruit skin at sunrise and sunset over three continuous days. HAS: hours after sunrise of day 1. The actual transcript abundance data of PbRVEs are listed in Supplementary Table S3. (c) The expression patterns of PbRVE1a, 1b, 7 and 8 in ‘Red Zaosu’ fruit skin over three continuous days. The gray boxes indicate nighttime. (d) Multiple sequence alignment of RVE proteins. RVE proteins were aligned using ClustalW. The DNA-binding domain is indicated by a horizontal gray bar. The phylogenetic analysis was constructed with the Neighbor-joining method (1000 replications bootstrap test and JTT model distribution) using MEGA 7.0. The protein sequences of the RVEs were obtained from the pear genome. The gene accession numbers are listed in Supplementary Table S1. Error bars represent SEs of the means (n = 3).