PAE attenuated histopathological alterations of the liver, spleen, and brain. (A) The histopathological changes of the liver, spleen, and brain (HE), the magnification of the liver and brain is 400, and spleen is 100; (B) the rate of binucleated hepatocytes; (C) spleen white pulp proportion; (D) surviving neurons of CA3. The black arrows show binucleated hepatocytes, ★ represents the white pulp area. The data are expressed as mean ± SEM, n = 6. ## p < 0.01 and ### p < 0.001 indicate significant difference compared with the control group; * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate significant difference compared with the d-gal-treated control group. C, control group, C received saline; M, d-gal model group, M received 150 mg/kg d-gal; V, 100 mg/kg VE group, it received 100 mg/kg VE + 150 mg/kg d-gal; P-3, 3 mg/kg PAE group, it received 3 mg/kg PAE + 150 mg/kg d-gal; P-10, 10 mg/kg PAE group, it received 10 mg/kg PAE + 150 mg/kg d-gal; P-30, 30 mg/kg PAE group, it received 30 mg/kg PAE + 150 mg/kg d-gal.