Figure 4.

Functional analysis of FoxA1-overexpressing KKU-213 cells and the control cells: (A) mRNA expressions (mean ± SD) of FoxA1 measured by real-time PCR (n = 2 per condition); (B) FoxA1 protein expressions of transfected and control cells by immunocytochemical detection; (C) cell proliferation activity (mean ± SD) measured by MTT assay (n = 5 per each time point per condition); (D) cell invasion activity (mean ± SD) of transfected and control cells (n = 5 per condition); and (E) hematoxylin-stained invaded cells under a light microscope in the cell invasion assay. * p < 0.05 analyzed by Student’s t test. All results were confirmed by three independent experiments. Scale bars are equal to 50 μm.