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. 2020 Mar 4;21(5):1738. doi: 10.3390/ijms21051738

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The immature APP isoforms accumulating under NSC48478 are acetylated. Total cell lysates were immunoprecipitated with an anti-APP antibody and then analysed for both APP (upper panel) and anti-acetylated lysine (lower panel) antibodies. Only the immature APP isoforms are acetylated (arrowheads). Beads alone indicate the negative control of the IP (immunoprecipitate). Asterisks (around 100 kDa) point to a specific band deriving from anti-acetylated lysine antibody hybridization. L: cell lysate; SN: supernatant.