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NH4Cl rescues inhibitor effects on APP subcellular localization. GT1 cells grown on coverslips were treated or not with the inhibitor NSC48478 in the presence or absence of NH4Cl. GT1 cells were subjected to immunofluorescence analysis by using anti-KDEL and anti-APP antibodies. Colocalization between APP and KDEL was then measured as indicated in the methods section. Plus +) and minus (-) indicate the presence or absence of NH4Cl. Scale bars, 10 μm.
