Table 3.
Matched ions of b series, with the MS/MS fragmentation profile of the penta- (m/z 993.5027) and exa- (m/z 828.0855) charged (acetylated) thymosin β4 (Tβ4), and matched ions of the y series, with the MS/MS fragmentation profile of the penta- (m/z 988.2166) and exa- (m/z 823.5936) charged (acetylated) thymosin β10 (Tβ10).
| Ions of b Series, (Acetylated) Thymosin β4 (Tβ4) | |||||
| m/z | Ion | z | Sequence | Error (Da) | Deconvoluted Mass |
| 774.9006 | b13 | 2 | .SDKPDMAEIEKFD.k [1xAcetyl] | 0.053 | 1547.696 |
| 946.616 | b16 | 2 | .SDKPDMAEIEKFDKSK.l [1xAcetyl] | 0.157 | 1890.9178 |
| 1067.2304 | b18 | 2 | .SDKPDMAEIEKFDKSKLK.k [1xAcetyl] | 0.182 | 2132.097 |
| 831.2934 | b21 | 3 | .SDKPDMAEIEKFDKSKLKKTE.t [1xAcetyl] | 0.197 | 2490.2895 |
| 355.2822 | b27 | 9 | .SDKPDMAEIEKFDKSKLKKTETQEKNP.l [1xAcetyl] | 0.096 | 3187.6722 |
| 850.3742 | b29 | 4 | .SDKPDMAEIEKFDKSKLKKTETQEKNPLP.s [1xAcetyl] | −0.069 | 3397.7732 |
| 936.3339 | b32 | 4 | .SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKE.t [1xAcetyl] | −0.152 | 3741.9428 |
| 869.5772 | b37 | 5 | .SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQE.k [1xAcetyl] | 0.132 | 4342.2255 |
| 1086.6825 | b37 | 4 | .SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQE.k [1xAcetyl] | 0.128 | 4342.218 |
| 1164.2402 | b40-NH3 | 4 | .SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQA.g [1xAcetyl] | 0.145 | 4669.4134 |
| 788.6117 | b41 | 6 | .SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAG.e [1xAcetyl] | −0.129 | 4726.4448 |
| 1182.801 | b41 | 4 | .SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAG.e [1xAcetyl] | 0.194 | 4726.4304 |
| 971.9887 | b42 | 5 | .SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGE.s [1xAcetyl] | −0.107 | 4855.48 |
| Ions of y Series, (Acetylated) Thymosin β10 (Tβ10) | |||||
| m/z | Ion | z | Sequence | Error (Da) | Deconvoluted Mass |
| 839.5505 | y14 | 2 | p.TKETIEQEKRSEIS. | 0.1142 | 1676.8726 |
| 592.5017 | y15 | 3 | l.PTKETIEwebQEKRSEIS. | 0.1909 | 1773.9327 |
| 1775.0578 | y15 | 1 | l.PTKETIEQEKRSEIS. | 0.1397 | 1790.9491 |
| 944.3809 | y16 | 2 | t.LPTKETIEQEKRSEIS. | −0.1239 | 1887.0094 |
| 663.8185 | y17 | 3 | n.TLPTKETIEQEKRSEIS. | 0.1304 | 1988.0643 |
| 994.9422 | y17 | 2 | n.TLPTKETIEQEKRSEIS. | −0.0864 | 1988.0572 |
| 1052.2492 | y18 | 2 | k.NTLPTKETIEQEKRSEIS. | 0.1992 | 2119.131 |
| 1244.6476 | y21 | 2 | t.QEKNTLPTKETIEQEKRSEIS. | −0.0005 | 2504.3272 |
| 863.8784 | y22 | 3 | e.TQEKNTLPTKETIEQEKRSEIS. | 0.0947 | 2605.3821 |
| 1295.2735 | y22 | 2 | e.TQEKNTLPTKETIEQEKRSEIS. | 0.1016 | 2605.3748 |
| 983.2832 | y25 | 3 | k.KTETQEKNTLPTKETIEQEKRSEIS. | 0.1044 | 2946.5364 |
| 1130.0686 | y29 | 3 | k.AKLKKTETQEKNTLPTKETIEQEKRSEIS. | 0.1194 | 3403.8786 |
| 1172.5977 | y30 | 3 | d.KAKLKKTETQEKNTLPTKETIEQEKRSEIS. | −0.0498 | 3531.9735 |
| 630.6308 | y32 | 6 | s.FDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | 0.1209 | 3777.0594 |
| 756.3638 | y32 | 5 | s.FDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | −0.0466 | 3777.0525 |
| 945.1581 | y32 | 4 | s.FDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | −0.1031 | 3777.0452 |
| 810.8174 | y35 | 5 | e.IASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | 0.1763 | 4048.2055 |
| 807.212 | y35-NH3 | 5 | e.IASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | −0.0238 | 4048.21 |
| 1059.3714 | y37 | 4 | m.GEIASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | −0.1943 | 4251.2934 |
| 744.6523 | y39-H2O | 6 | p.DMGEIASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | −0.07 | 4480.3443 |
| 1116.7269 | y39-H2O | 4 | p.DMGEIASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | 0.1471 | 4479.3502 |
| 916.6534 | y40 | 5 | k.PDMGEIASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | 0.1754 | 4594.421 |
| 965.1255 | y42 | 5 | a.DKPDMGEIASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | 0.0231 | 4837.5425 |
| 1206.0731 | y42 | 4 | a.DKPDMGEIASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | −0.053 | 4837.5354 |
| 801.7036 | y42-NH3 | 6 | a.DKPDMGEIASFDKAKLKKTETQEKNTLPTKETIEQEKRSEIS. | 0.1215 | 4820.5236 |