Figure 1.

Schematic diagram of the experiment. Human neuroblostoma cells, SH-SY5Y, were incubated with normal medium or medium with 100 nM insulin for 48 h to induce insulin resistance. After 48 h, the cells without insulin induction were further treated with normal medium (C) or 500 nM liraglutide (L), while the insulin-resistant cells were further treated with 100 nM insulin (I) or 500 nM liraglutide (IL) for 24 h. Before harvesting cells, each group was subdivided into 2 groups (stimulated with 10 nM insulin or left unstimulated for 30 min). D0, D1, D2, and D3 represent the experimental days as day0, day1, day2, and day3, respectively, after cell seeding.