Table 1.

Primers used in end-point PCR and quantitative PCR based on SYBR^® Green chemistry.

Primers	Primer Sequences	Amplicon Size
	GAPDH Primers
GAPDH1	5′AACGGATTTGGTCGTATTGGGC3′	600 bps
GAPDH2	5′AGGGATGATGTTCTGGAGAGCC3′
GAPDH2	5′AGGGATGATGTTCTGGAGAGCC3′	644 bps
GAPDH3	5′AAGCTGACTCAGCCCGCAAAGG3′
	PKM Primers
PKM1 F	5′TCCACAGACCTCATGGAAGC3′	157 bps
PKM1 R	5′GATTCCGGGTCACAGCAATG3′
PKM2 F	5′GAGGCCTCCTTCAAGTGCTG3′	231 bps
PKM2 R	5′CATGGCAAAGTTCACCCGGA3′
PKM(C) F	5′TCAGATGCTGGAGAGCATGAT3′*	442 bps
PKM(C) R	5′CTTGCACAGCACAGGGAAGATGCC3′
	PHGDH Primers
PHGDHSYF	5′AAAGAGGAGCTGATAGCGGA3′
PHGDHSYR	5′ACCACCTGGAGTTTCTCAGC3′	107 bps
	SHMT2 Primers
SHMT2/SYF	5′CCAGGTCAGGGCTCATCTTC3′	169 bps
SHMT2/SYR	5′TGCTTTAGGGCCACAGCTAC3′
	Housekeeping Genes (SYBR Green)
HMBSF	5′GGCAACTGTACCTGACTGGA3′	110 bps
HMBSR	5′CTCAGGGCCATCTTCATGCT3′
B-actinF	5′GAGCACAGAGCCTCGCCTT3′	196 bps
B-actinR	5′CCCACCATCACGCCCTGG3′
PPIAF	5′GACTGAGTGGTTGGATGGCA3′	77 bps
PPIAR	5′GCTCCATGGCCTCCACAATA3′

^* underlined: 3′exon 7; italic: 5′ exon 8