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. 2020 Feb 28;21(5):1655. doi: 10.3390/ijms21051655

Figure 5.

Figure 5

The effect of IGF2BP3 on the proliferation of SMSCs. (A,B) Screening IGF2BP3 interfering fragments and verifying overexpression efficiency by qPCR and Western Blot. The data are presented as mean ± SEM (n. = 6). The Student’s t-test was used to compare expression levels among different groups. * p < 0.05; ** p < 0.01 vs. NC. (C,D) Cell growth curves, as measured from CCK-8 assays, following transfection with si-IGF2BP3, pcDNA3.1-IGF2BP3, or negative control in SMSCs. (E,F) Cell cycle analysis of SMSCs 48 h after the overexpression and inhibition of IGF2BP3. (G) The results of the EdU assay for SMSCs transfected with si-IGF2BP3, pcDNA3.1-IGF2BP3, or negative control, where EdU (red) fluorescence is used as an indicator of proliferation and nuclei are indicated by DAPI (blue) fluorescence. Micrographs were taken using 200× magnification, and the proliferation rates of SMSCs cells were measured with IGF2BP3 overexpression and inhibition. The data are expressed as mean ± SEM (n. = 6). * p < 0.05; ** p < 0.01 vs. NC.