Table 1.
Name | Sequence (5′-3′) |
---|---|
miR-9-5p mimic | UCUUUGGUUAUCUAGCUGUAUGA |
Negative mimic | UUGUACUACACAAAAGUACUG |
miR-9-5p inhibitor | UCAUACAGCUAGAUAACCAAAGA |
Negative inhibitor | CAGUACUUUUGUGUAGUACAA |
Si-IGF2BP3-984 | GCUGCUGAGAAACCAAUUATT UAAUUGGUUUCUCAGCAGCTT |
Si-IGF2BP3-1214 | GCAGGACUUGACACUCUAUTT AUAGAGUGUCAAGUCCUGCTT |
Si-IGF2BP3-1519 | CCUUGGCAGUUGGAGCUAUTT AUAGCUCCAACUGCCAAGGTT |
Si-NC | UUCUUCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT |
For IGF2BP3 overexpression plasmid construction, the coding sequence fragments of IGF2BP3 was cloned into pcDNA3.1 (+) vector using T4 DNA ligase (TakaRa; Tokyo, Japan) following the restrictive endonuclease digestion of HindIII and KpnI restriction enzyme (TakaRa). Then, the successfully constructed vectors were termed as pcDNA3.1- IGF2BP3 after confirming by sequencing.