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. 2020 Feb 28;21(5):1655. doi: 10.3390/ijms21051655

Table 1.

RNA oligonucleotides in this article.

Name Sequence (5′-3′)
miR-9-5p mimic UCUUUGGUUAUCUAGCUGUAUGA
Negative mimic UUGUACUACACAAAAGUACUG
miR-9-5p inhibitor UCAUACAGCUAGAUAACCAAAGA
Negative inhibitor CAGUACUUUUGUGUAGUACAA
Si-IGF2BP3-984 GCUGCUGAGAAACCAAUUATT
UAAUUGGUUUCUCAGCAGCTT
Si-IGF2BP3-1214 GCAGGACUUGACACUCUAUTT
AUAGAGUGUCAAGUCCUGCTT
Si-IGF2BP3-1519 CCUUGGCAGUUGGAGCUAUTT
AUAGCUCCAACUGCCAAGGTT
Si-NC UUCUUCGAACGUGUCACGUTT
ACGUGACACGUUCGGAGAATT

For IGF2BP3 overexpression plasmid construction, the coding sequence fragments of IGF2BP3 was cloned into pcDNA3.1 (+) vector using T4 DNA ligase (TakaRa; Tokyo, Japan) following the restrictive endonuclease digestion of HindIII and KpnI restriction enzyme (TakaRa). Then, the successfully constructed vectors were termed as pcDNA3.1- IGF2BP3 after confirming by sequencing.