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. 2020 Mar 3;21(5):1722. doi: 10.3390/ijms21051722

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pretreatment with elf18 peptide reduced transient transformation efficiency in wild-type, RTNLB4 O/E transgenic, and three rtnlb4 mutant seedlings. (A) Transient transformation efficiencies of wild-type seedlings pretreated with 10 µM elf18 peptide for 0, 6, or 24 hr before infection with A. tumefaciens. Transient transformation rates of wild-type, efr-1, fls2, and bak1 mutants (B), RTNLB4 O/E transgenic plants (C), and three rtnlb4 mutant plants (D) pretreated with 10 µM elf18 peptide for 6 h before infection with A. tumefaciens. Distilled H₂O (dH₂O) was used as the mock control in seedling transient transformation assays. Data are mean ± SE from at least three independent transformation assays. Data were analyzed by Duncan tests and means with different letters were significantly different (p < 0.05).