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. 2020 Mar 3;21(5):1722. doi: 10.3390/ijms21051722

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transient transformation rates of wild-type, RTNLB4 O/E transgenic and three rtnlb4 mutant seedlings were decreased with VirB2 peptide pretreatments. (A) Transient transformation rates of wild-type seedlings pretreated with 1, 5, 10, 20, or 50 µM of five VirB2 peptides, S111-T58, I63-I80, I80-V101, G95-F112, or I104-G121, for 6 hr before infection with A. tumefaciens. (B) Transient transformation efficiency of wild-type seedlings pretreated with 10 µM of two VirB2 peptides, S111-T58 or I63-I80, at 0, 6, or 24 hr before infection with A. tumefaciens. Agrobacterium-mediated transient transformation rates of wild-type, efr-1, fls2, bak1 mutants (C), RTNLB4 O/E transgenic plants (D), and three rtnlb4 mutant plants (E) pretreated with 10 µM of the two VirB2 peptides for 6 hrs before infection with A. tumefaciens. DMSO solution was used as the mock control in seedling transient transformation assays. Data are mean ± SE from at least three independent transformation assays. Data were analyzed by Duncan tests and means with different letters were significantly different (p < 0.05).