Figure 8.

Induced H₂O₂ amounts were lower in RTNLB4 O/E transgenic plants and rtnlb4, efr-1, and fls2 mutants than in wild-type plants after with elf18 and VirB2 peptides. The H₂O₂ amount in wild-type, RTNLB4 O/E transgenic plants (A) and three rtnlb4, efr-1, and fls2 mutant plants (B) was determined at 0, 0.5, 2, 5, and 10 min after the addition of elf18. The H₂O₂ amount at each time was normalized to the H₂O₂ amount at 0 min. H₂O₂ amount in wild-type, RTNLB4 O/E transgenic (C,E) and three rtnlb4, efr-1, and fls2 mutant plants (D,F) was determined after adding two VirB2 peptides S111-T58 (C,D) or I63-I80 (E,F). Data are mean ± SE from more than 10 plants. Data were analyzed by Duncan tests and means with different letters were significantly different (p < 0.05).