. 2020 Feb 28;21(5):1641. doi: 10.3390/ijms21051641

Table 5.

L-arginine/NO pathway-associated enzymes in inflamed and quiescent large bowel.

Gene	CD_i (n = 9)	CD_q (n = 16)	UC_i (n = 15)	Normal (n = 20)	p-value
ARG1 [NRQ]	0.79 (0.5–28.7) ^a	1.18 (0.5–7.2) ^a	8.86 (1.3–15.4) ^a	0 (0–0) ^b–d	<0.0001
ARG2 [NRQ]	0.96 (0.6–6.4) ^a	1.4 (0.8–3.0) ^a	1.45 (0.7–2.4) ^a	0 (0–0) ^b–d	<0.0001
DDAH1 [NRQ]	0.48 (0.1–2.9) ^c	2.75 (0.9–4.7) ^a,b,d	0.72 (0.2–2.5) ^c	0.3 (0.2–0.6) ^c	0.002
DDAH2 [NRQ]	1.57 (1.2–3.1) ^a,d	1.54 (1.2–1.9) ^a,d	0.93 (0.8–1.3) ^a–c	0.31 (0.1–0.4) ^b–d	<0.0001
NOS2 [NRQ]	0.21 (0.05–1.2) ^a,d	0.74 (0.2–1.5) ^a,d	7.96 (1–24.2) ^a–c	0.07 (0.04–0.1) ^b–d	<0.0001
ODC1 [NRQ]	1.27 (0.9–2.1) ^a,c	2 (1.6–2.3) ^a,b	1.72 (1.4–2.3) ^a	0.02 (0.01–0.03) ^b–d	<0.0001
PRMT1 [NRQ]	1.68 (0.8–3.7) ^a	1.76 (1.3–2) ^a	1.33 (1.2–1.8) ^a	0.08 (0.05–0.2) ^b–d	<0.0001
PRMT2 [NRQ]	1.55 (1–5.4) ^a	1.7 (1.4–3.1) ^a,d	1.42 (0.9–1.7) ^a,c	0 (0–0) ^b–d	<0.0001
PRMT5 [NRQ]	1.09 (0.9–3) ^a	1.7 (1.5–2) ^a	1.54 (1.4–1.8) ^a	0.07 (0–0.2) ^b–d	<0.0001

Data were normally distributed following log-transformation and are presented as geometric means of normalized relative quantities (NRQ) with 95% confidence interval and analyzed using one-way ANOVA. ARG, arginase; DDAH, dimethylarginine dimethylaminohydrolase, NOS, nitric oxide synthase; ODC, ornithine decarboxylase; PRMT, arginine N-methyltransferase; CD_i, inflamed tissue obtained from patients with Crohn’s disease; CD_q, quiescent tissue obtained from patients with Crohn’s disease; UC_i, inflamed tissue obtained from patients with ulcerative colitis; Normal, normal large bowel tissue obtained from patients undergoing polypectomy; ^a significantly different from normal; ^b significantly different from CD_i; ^c significantly different from CD_q; ^d significantly different from UC_i.