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. 2020 Mar 6;21(5):1821. doi: 10.3390/ijms21051821

Figure 1.

Figure 1

Effect of Bisphenol S (BPS) on human granulosa cell (HGC) viability. HGC underwent 48-h culture in the presence or absence of BPS (10 nM, 100 nM, 1 µM, 10 µM or 50 µM). (A.) HGC viability was assessed using the Live/Dead Viability/Cytotoxicity Kit for mammalian cells. The results of three independent experiments per condition are expressed as the percentage of living cells in each condition. (B.) HGC viability was assessed on spent 48-h culture media using the Bioluminescence Cytotoxicity Assay Kit. Data are expressed as the mean relative light units ± standard error of the mean (SEM) of six independent cultures. Bars with different superscripts indicate a significant difference (p ≤ 0.05).