Figure 3.

Effect of Bisphenol S (BPS) on human granulosa cell (HGC) progesterone secretion. HGC underwent 48-h culture in the presence or absence of BPS (10 nM, 100 nM, 1 µM, 10 µM or 50 µM). The progesterone concentration was measured in culture media, and its value was normalised by the protein concentration in each well. Data are expressed as ng progesterone per µg protein and normalised to the control condition. The results of seven independent experiments are presented as the mean ± standard error of the mean (SEM). Bars with different superscripts indicate a significant difference (p ≤ 0.05).