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. 2020 Mar 6;21(5):1821. doi: 10.3390/ijms21051821

Figure 6.

Figure 6

Effect of BPS on HGC gene expression. The expression of six hormonal receptors (oestrogen receptors (A): oestrogen receptor 1 [ESR1], oestrogen receptor 2 [ESR2], Oestrogen-related receptor γ [ESRRG] and G protein-coupled oestrogen receptor [GPER] and (B) androgen receptor [AR], progesterone receptor [PR]), and two genes involved in steroidogenesis (B: CYP17A1 and StAR), was determined in HGC after 48-h culture in complemented serum-free McCoy’s 5A media in the presence or absence of BPS (10 nM, 100 nM, 1 µM, 10 µM or 50 µM). Total messenger RNA (mRNA) was extracted from HGC and reverse transcribed, and real-time polymerase chain reaction (qPCR) was performed. The geometric mean of two housekeeping genes (Glyceraldehyde-3-phosphate dehydrogenase [GAPDH] and ribosomal protein L19 [RPL19]) was used to normalise gene expression. The results are expressed as mean ± standard error of the mean (SEM) of six independent cultures and normalised to the mean of control condition. No letter in common indicates a significant difference (p < 0.05).