Table 2.
Characteristics of primary antibodies used for western blotting.
| Name | Species Specificity | Source | Supplier (Distributor, Town, Country) | Catalogue Number | Primary Ab Dilution |
|---|---|---|---|---|---|
| MAPK3/1 (ERK1/2) | Rat | Rabbit polyclonal antibody | Cell Signaling Technology (Ozyme, Saint Quentin Yvelines, France) | 9102 | 1/1000 |
| Phospho-MAPK3/1 (ERK1/2; Thr202/Tyr204, D13.14.4E, XPTM) | Human | Rabbit monoclonal antibody | Cell Signaling Technology | 4370 | 1/2000 |
| CYP19A1 | Human | Rabbit polyclonal antibody | Sigma Aldrich (Saint Louis, Missouri, USA) | SAB1410280X | 1/500 |
| CYP11A1 | Human | Goat polyclonal antibody | Santa Cruz Biotechnology (Dallas, USA) | sc-18043 | 1/500 |
| HSD3B1 | Human | Rabbit polyclonal antibody | Abgent (San Diego, USA) | AP14585a | 1/500 |
| Vinculin | Human | Mouse monoclonal antibody | Sigma Aldrich (Saint Quentin Fallavier) | V9131 | 1/1000 |