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. 2020 Mar 4;21(5):1756. doi: 10.3390/ijms21051756

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Specificity of the LAMP assay for the detection of CuLCrV in the mix virus assay. CuLCrV was detected from mixed virus DNA with virus-specific primers using PCR (A). LAMP amplified products were analyzed using agarose gel electrophoresis (B), and real-time amplification with Genie^® III (C). Here, we used Virus Mix No. 1: CuLCrV with other viruses including SLCV and TYLCV, Virus Mix No. 2: CuLCrV with the healthy control, Virus Mix No. 3: mix viruses, i.e., SLCV and TYLCV excluding CuLCrV with healthy control, HC: healthy squash leaf as the healthy control, Neg: DH₂0 as the negative control, and M: 100 bp DNA ladder (A,B). Successful LAMP amplification is demonstrated by the curve amplified using Genie^® III (C).