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. 2020 Mar 4;21(5):1756. doi: 10.3390/ijms21051756

Figure 6.

Figure 6

Detection of CuLCrV from symptomatic (A–E) and asymptomatic infected (F,G,H,I,J) cucumber and winter squash leaf samples using LAMP (B–D,G–I), PCR (A,F) and qPCR (E,J). LAMP amplified products were analyzed using agarose gel electrophoresis (B,G), and visual inspection with SYBR™ green 1 DNA gel staining, where positive amplification gave a fluorescent green color and negative reactions remained orange under UV light (C,H), and real-time amplification with Genie® III (D,I). CuLCrV titer was quantified using qPCR relative to internal control gene Rubisco (E,J). Here, we used TC-Sy1, 2: Tift county cucumber symptomatic infected leaf sample no. 1,2; LC-Sy1: Lowndes county cucumber symptomatic infected sample no. 1; TWS-Sy1, 2: Tift county winter squash symptomatic infected leaf sample no. 1,2; LWS-Sy1: Lowndes county winter squash symptomatic infected sample no. 1; TC-AI1, 2: Tift county cucumber asymptomatic infected leaf sample no. 1,2; LC-AI1: Lowndes county cucumber asymptomatic infected sample no. 1; TWS-AI1, 2: Tift county winter squash asymptomatic infected leaf sample no. 1,2; LWS-AI1: Lowndes county winter squash asymptomatic infected sample no. 1; HC: healthy control, Neg: negative control or non-template control.