Figure 1.

Effect of ethanol extracts of Psoralea corylifolia (EPC) on myogenesis. C2C12 cells were supplemented with differentiation medium (DM), including the indicated concentrations of EPC (1, 10, 100 and 1000 ng/mL) for 3 days, and then cells were collected to perform (A) immunostaining of MHC (red) and DAPI (blue) (scale bar = 200 µm) and (B) Western blot analysis to determine the level of myosin heavy chain (MHC) expression. The level of MHC protein was quantified and normalized to pan-cadherin. Values are mean (n = 3). The images are representative of three independent experiments with similar results. Means without a common superscript differ significantly (p < 0.05).