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. 2020 Feb 25;21(5):1571. doi: 10.3390/ijms21051571

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of corylifol A on myogenesis. C2C12 cells were differentiated in the presence of corylifol A (CA, 10, 50 and 100 nM) for 3 days, and then cells were collected to perform (A) immunostaining of MHC (red) and DAPI (blue) (scale bar = 200 µm) and (B) Western blot analysis to determine the expression of myogenic markers. Values are mean (n = 3). (C) C2C12 myoblasts were transfected with a MyoD-responsive reporter 4RTK-luciferase, and then cells were differentiated in the presence of corylifol A, followed by measurement of luciferase activity. Data are expressed as mean ± S.D. of three independent experiments. (D) C2C12 myoblasts treated with corylifol A (100 nM) and harvested on differentiation day 1 (D1), 2 (D2) and 3 (D3) to evaluate the expression of myogenic markers. Values are mean (n = 3). The images are representative of three independent experiments with similar results. Means without a common superscript differ significantly (p < 0.05).

Effect of corylifol A on myogenesis. C2C12 cells were differentiated in the presence of corylifol A (CA, 10, 50 and 100 nM) for 3 days, and then cells were collected to perform (A) immunostaining of MHC (red) and DAPI (blue) (scale bar = 200 µm) and (B) Western blot analysis to determine the expression of myogenic markers. Values are mean (n = 3). (C) C2C12 myoblasts were transfected with a MyoD-responsive reporter 4RTK-luciferase, and then cells were differentiated in the presence of corylifol A, followed by measurement of luciferase activity. Data are expressed as mean ± S.D. of three independent experiments. (D) C2C12 myoblasts treated with corylifol A (100 nM) and harvested on differentiation day 1 (D1), 2 (D2) and 3 (D3) to evaluate the expression of myogenic markers. Values are mean (n = 3). The images are representative of three independent experiments with similar results. Means without a common superscript differ significantly (p < 0.05).