Figure 5.

Protective effects of corylifol A against dexamethasone-induced myotube atrophy in vitro. C2C12 myoblasts were differentiated for 3 days, followed by treatment with dexamethasone (Dex, 1 µM) along with vehicle DMSO or corylifol A (CA, 100 nM) for an additional 48 h. (A) Collected cells were fixed and immunostained with MHC (red) and DAPI (blue) (scale bar = 200 µm). (B) Cell lysates were subjected to Western blot analysis to analyze phosphorylated NF-κB, E3 ligases, myostatin, phosphorylated Akt and MHC. Values are mean (n = 3). The images are representative of three independent experiments with similar results. Means without a common superscript differ significantly (p < 0.05).