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. 2020 Mar 3;21(5):1733. doi: 10.3390/ijms21051733

Figure 5.

Figure 5

Impact of miR-24-3p inhibition on Notch pathway components’ miR-24-3p expression in cultured HUVECs and microvascular ECs exposed to limb ischemia. (A) Bar graph shows miR-24-3pNotch-1, Dll1, and down-stream targets Hes-1 and Hey-1 mRNAs in HUVEC transfected with miR-24-3p inhibitor (light grey columns) compared to control (white columns). (B) Bar graph shows the modulations of Notch-1, Dll1, Hes-1, and Hey-1 mRNA in CD146 positive cells isolated by ischemic adductor muscle at 3 days post-ischemia treated with in vivo miR-24-3p inhibitor (Ad.Decoy.miR-24-3p, grey bar) compared to scramble control (Ad. Null, black bar). Gene expression was normalised to 18S. Cell experiments were performed in triplicate and repeated three times. Analyses on muscular cells were developed using n = 10 mice per group. Values are means ± SEM. ** p < 0.005 vs. the respective control.