Skip to main content
. 2020 Mar 4;21(5):1747. doi: 10.3390/ijms21051747

Figure 3.

Figure 3

FoxA2 is a PPARβ/δ target gene in skeletal muscle. (A,B) Relative FoxA2 mRNA (A) and protein expression (B) in myotubes treated for the indicated time with indicated concentrations of GW501516 (GW). Representative immunoblots of FoxA2 are shown. Actin serves as a loading control from the same samples. Values are the mean + SD from 3 independent experiments. * p < 0.05; ** p < 0.01; and n.s., not significant; (C) A schematic diagram of putative PPREs in the promoter and the first intronic region of mouse FoxA2 gene. The below table shows the putative PPRE sequences and scores, with a maximum score of 1.0, which reflect the predicted confidence using NUBIScan, a prediction software for nuclear receptor response elements; (D,E) Chromatin immunoprecipitation (ChIP) of predicted PPREs in FoxA2 gene with PPARβ/δ antibody or pre-immune IgG (p.i.) in C2C12 treated with either vehicle (Veh) or GW (D). Quantitative ChIP-qPCR analysis of PPARβ/δ binding at PPRE1 in the presence or absence of GW treatment (E); (F) Relative luciferase activity from reporter gene constructs containing either PPRE1 or ΔPPRE1 in HEK293T cells in the presence or absence of 1 and 10 µM GW for 6 h. DMSO was used as the vehicle control. Values are the mean + SD from 3 independent experiments. * p < 0.05; ** p < 0.01; and n.s., not significant.