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. 2020 Mar 3;21(5):1718. doi: 10.3390/ijms21051718

Figure 2.

Figure 2

Ikaros represses RAB20 transcription. (A) Human B-ALL cell lines were transduced with empty vector (MIG-CTL) or Ikaros (MIG-IKZF1) and relative IKZF1 expression was determined by qRT-PCR. (B) qChIP was used to determine Ikaros occupancy at the RAB20 promoter after Ikaros overexpression. (C) Ikaros overexpression resulted in a decrease in RAB20 expression as determined by qRT-PCR. (D) Human B-ALL cell lines were transduced with scrambled shRNA (shCTL) and shRNA targeting IKZF1 (shIKZF1) and relative Ikaros expression determined using qRT-PCR. (E) qChIP was used to determine Ikaros occupancy at the RAB20 promoter after Ikaros knockdown. (F) Ikaros knockdown resulted in an increase in RAB20 expression as determined by qRT-PCR. (G) The effect of Ikaros on transcription from the URE of human RAB20 using a luciferase reporter assay following transfection with control vector (pcDNA3.1-CTL) or human Ikaros (pcDNA3.1-IKZF1). The graphed data are the mean ± SD (error bars) from three independent experiments.